Although religious involvement and spirituality have been considered significant factors for wellbeing and resilience, ministry workers are considered an at-risk population due to high levels of occupational stress. Experiencing levels of burnout similar to those associated with teachers and social workers, the mental health of ministers affects not only themselves but also the wellbeing of their families, those in their immediate ministry, as well as the church networks and communities in which they serve. Strengthening individual psychological resilience requires an understanding of the contextual stressors that shape ministry work. The Ministry Stressors Framework presented in this paper reveals key hazards across four categories that will guide efforts to strengthen resilience in ministry practice. Using a sequential, mixed-method design, this paper asks what types of stressors Australian ministry workers experience and how stressful they find them, with the aim of providing a framework for future research. We propose that four categories of ministry hazards will be apparent: (1) workload complexity stressors, (2) emotional load stressors, (3) conflict stressors, and (4) personal or family stressors. Quantitative data were collected in a cross-sectional study assessing the prevalence and impact of 40 contextualised stressor events representing the four categories in 277 Australian ministry workers. Qualitative data were separately collected from 119 Australian ministry workers, classifying their experiences against, and broadening, the list of 40. Although workload complexity and emotional load stressors were most prevalent, they were perceived to have lower impact. Personal stressors had high impact but were typically less prevalent. However, conflict stressors were moderately high in both prevalence and impact. Findings suggest a need to strengthen ministry workers’ resilience in the face of conflict and tension within their teams and beyond, and to provide preventative mental health interventions for this unique but understudied vocational group.